Bbiochemtools

Amino Acid Quiz: Master All 20

The fastest way to lock in the 20 standard amino acids: one-letter codes, three-letter codes, polarity, and charge. Free, no signup. Built for biochem, MCAT, and pre-med.

Question 1 of 10Score: 0

What is the one-letter code for

Glycine?

The 20 standard amino acids: reference table

Use this chart alongside the quiz. The one-letter codes are what trip most students up, so the quiz drills those first.

Name3-letter1-letterClassCharge

*His is the one to watch: its imidazole side chain has a pKa of about 6.0, so at physiological pH 7.4 it's only around 10% protonated, reliably "basic" as a class, but not reliably charged like Lys and Arg. That gap is exactly why it's a favorite MCAT trick answer. See the amino acid titration curve tool for the full breakdown.

How to memorize the amino acids fast

Most students try to memorize all 20 at once and burn out. The trick is to group them by class first, nonpolar, polar, acidic, basic, then drill the one-letter codes separately, because those are nearly random and need pure repetition. This quiz is built around exactly that order. Run "Name → class" until it's automatic, then switch to "Name → 1-letter code."

More free tools: all biochem tools · peptide charge & pI calculator · enzyme kinetics simulator

How the 20 amino acids break down by class

Grouping first is faster than memorizing 20 flat facts. The 20 standard amino acids split into exactly 5 classes:

Nonpolar7, Gly, Ala, Val, Leu, Ile, Pro, Met
Aromatic3, Phe, Trp, Tyr
Polar5, Ser, Thr, Cys, Asn, Gln
Basic3, Lys, Arg, His
Acidic2, Asp, Glu

7 + 3 + 5 + 3 + 2 = 20. Nonpolar is the biggest group by far, which is why "is it nonpolar?" is the single most useful first question when you're unsure of an amino acid's class.

The 9 one-letter codes that don't match the first letter

Most codes are just the first letter of the name (Gly→G, Ala→A, Val→V...). These 9 are the exceptions, and they're exceptions for a consistent reason: their "obvious" letter was already taken by another amino acid.

Phenylalanine → FP already taken by Proline
Tryptophan → WT already taken by Threonine
Tyrosine → YT already taken (twice over, by Threonine and Trp's claim)
Asparagine → NA already taken by Alanine
Glutamine → QG already taken by Glycine
Lysine → KL already taken by Leucine
Arginine → RA already taken by Alanine
Aspartate → DA already taken by Alanine
Glutamate → EG already taken by Glycine

Three names start with A (Alanine, Asparagine, Aspartate) and three start with G (Glycine, Glutamine, Glutamate), which is exactly why so many of the exceptions cluster around those two letters.

FAQ

Why don't all codes match the first letter of the name?
Three names start with A, three start with G, only one of each group could keep the obvious letter. The rest got distinctive alternates, listed in the table above.

Is side chain charge always fixed, or does it depend on pH?
The charges here assume physiological pH (~7.4). Technically it depends on the side chain's pKa relative to the surrounding pH. See the peptide charge calculator to compute it at any pH.

Why memorize class before the one-letter codes?
Class sorts 20 amino acids into 5 groups with clear chemical logic, far easier to reason through than 20 arbitrary letters, which benefit more from pure repetition once classes are automatic.

Why "Aspartate/Glutamate" instead of "Aspartic acid/Glutamic acid"?
Same molecule, different naming convention. At physiological pH the acidic side chain is deprotonated (ionized), so biochemists typically use the "-ate" name for the ionized form, the same pattern as lactate vs. lactic acid.

Want the by-hand method for pI? See how to calculate pI without a calculator.

Related tools: Peptide charge & pI calculator · Amino acid titration curve · Amino acid catabolism explorer · Amino acid chart (all 20) · all biochem tools.