How to translate DNA to protein by hand
First transcribe: the mRNA has the same sequence as the DNA coding strand, except every thymine (T) becomes uracil (U). Then read the mRNA in non-overlapping groups of three bases called codons. Translation begins at the first start codon (AUG, which codes for methionine) and continues until a stop codon (UAA, UAG, or UGA), which codes for no amino acid and ends the protein. Each codon maps to one amino acid via the genetic code below. The reading frame matters — shifting the start by one base changes every codon downstream.
Note: this tool reads the coding (sense) strand in the 5′→3′ direction and uses the standard genetic code. It translates the full sequence in the chosen frame; in real cells, ribosomes begin at the first AUG.
The genetic code (mRNA codons)
Related tools: Peptide charge & pI calculator · Amino acid quiz · all biochem tools.